SSB and Nova have collaborated to develop the ambr 15 automated micro bioreactor system combined with BioProfile FlEX2 automated cell culture analyser
SSB ambr 15 bioreactor system
This unique integrated system, developed by the collaboration of Sartorius Stedim Biotech (SSB) and Nova Biomedical (Nova), enables rapid at line sample collection and analysis of massive quantities of key cell culture data, shown for the first time at the Cell Line Development & Engineering Conference in San Diego, California.
SSB is an international supplier of products and services, aiming to assist the biopharmaceutical industry develop and manufacture drugs safely and efficiently.
Nova works to develop and manufacture state of the art, whole blood, point of care and critical care analysers, as well as providing the biotechnology industry with advanced instruments for cell culture monitoring.
Matthew McRae, Biotechnology Sales Product Line Manager at Nova, said: “There is a significant demand for fully automated cell culture analysis of small-volume culture systems like the Sartorius ambr micro bioreactor system.
"We’re excited to have worked with SSB on this integration program because our BioProfile FLEX2 with maintenance-free sensors provides rapid analysis of key cell culture tests using just 265 microliters of sample. As a result, this analyser is a highly complementary addition to the ambr 15 while maintaining the flexibility and modularity of the first-generation BioProfile FLEX analyser.”
Nova BioProfile FLEX2 automated cell culture analyser
The system developed by SSB and Nova is said by the collaboration to seamlessly integrate the software interface that controls the ambr 15 workstation, for automated parallel processing of 24 or 48 single-use bioreactors, (10–15ml working volume) with the cell culture analysis capability of the innovative BioProfile FLEX2.
The combined system provides a unique tool to simultaneously run, sample and analyse a massive number of cell culture conditions during cell line and media development by design of experiments (DoE).
Combining the ambr 15 and the BioProfile FLEX2 enables fully integrated automatic sample transfer, analysis and automated feedback control in each single use ambr 15 bioreactor.
This allows collection of enormous quantities of cell culture data, including total and viable cell density, viability, cell diameter, pH, PCO2, PO2, glucose, lactate, glutamine, glutamate, ammonium, Na+, K+, Ca++ and osmolality, which can be sampled and measured with a complete cycle time of just 6–7 minutes.
The ambr 15 software simultaneously acquires the BioProfile FLEX2 data set and processes it by performing complex in run calculations of for example, how much feed volume to automatically add during a run. This saves time by significantly increasing the amount of consistent clone selection, media development and culture parameter data that a scientist can generate per run.
Dr. Barney Zoro, ambr Product Manager at SSB, said: “We have combined two highly innovative technologies. Our successful partnership with Nova has delivered complete at line analytics capabilities for our ambr 15 platform. Utilising these integrated systems will support scientists to more easily run full quality by design (QbD) studies, without causing a sampling bottleneck or needing additional staff resources. The new system will optimise their process productivity, as well as the quality of their biologics.”