GE Healthcare's Biotin CAPture kit is for the fast and convenient capture of a biomolecule to a sensor chip surface and subsequent analysis in Biacore systems, including unstable molecules or those for which no regeneration protocol can be established.
An alternative to direct immobilisation or biotin-streptavidin immobilisation, the Biotin CAPture kit features a standardised protocol for regeneration, and the ability to change ligands between experiments without changing the sensor chip.
The kit allows for the reversible capture of biotinylated molecules and generic regeneration. The sensor chip, Sensor Chip CAP, is built on a carboxymethylated dextran matrix to which an ss-DNA molecule is pre-immobilised. Biotin CAPture Reagent consists of streptavidin conjugated with the complementary ss-DNA molecule.
The kit is available in two versions: Biotin CAPture kit and Biotin CAPture kit series S, one for each sensor chip format used in Biacore systems. They are compatible with most samples and the majority of applications and analyses performed using Biacore systems.
The Biacore X100 2.0, due to be launched in September, includes a built-in pre-programmed workflow to support the kit.