Higher multiplexing system for gene expression
Beckman Coulter has launched its new GenomeLab GeXP Genetic Analysis System.
Beckman Coulter has launched its new GenomeLab GeXP Genetic Analysis System.
Providing high-throughput, quantitative gene expression through scalable, multiplexed PCR, the system also employs its patented priming strategy, a two-primer process with gene-specific universal primers, to overcome the biases (primer-primer reactions; primer-product cross-reactions) that limit standard multiplexed PCR analysis to 'just a few genes at a time'. Additionally, this priming strategy, combined with single endpoint fluorescence detection, enables higher levels of multiplexing.
The system delivers more genes per reaction and more samples per run, removing bottlenecks in gene expression studies for drug discovery and development research. It can run two 96-well plates in 24 hours and examine the expression of 20 to 35 genes in a single reaction per well.
Most suited to applications working with smaller gene sets, the system is cost-effective, and can deliver accurate and quantitative results for hundreds or thousands of samples using very small amounts of total RNA.
The system includes a series of software tools for automated primer design and multiplex assembly, reaction plate setup, data collection, data checking and first-pass analysis. It also provides genetic analysis functions, including sequencing, heterozygote detection, SNP detection, STR, visualisation, AFLP and LOH. Sample preparation and reaction setup and cleanup can be performed on Beckman's Biomek 3000 Laboratory Automation Workstation.
A new line of consumables for the GeXP includes reagents for discovery, master mix reagents for user-defined genes and multiplex kits for performing quantitative gene expression studies on human, mouse or rat genes. Exisiting CEQ 8000 and 8800 genetic analysis systems can be upgraded to GeXP capabilities.