Beckman Coulter Genomics collaborates on validation of SPRIworks

Automated process enhances speed and reproducibility

The SPRIworks Fragment Library System I utilises Solid Phase Reversible Immobilization (SPRI) paramagnetic bead-based technology

Beckman Coulter has announced a collaboration with the J. Craig Venter Institute (JCVI) on the validation of the SPRIworks Fragment Library System I for the Illumina Genome Analyser. The automated benchtop system prepares up to 10 DNA libraries in five hours with high reproducibility and consistency.

The SPRIworks Fragment Library System I, developed by Beckman Coulter Genomics, utilises Solid Phase Reversible Immobilisation (SPRI) paramagnetic bead-based technology. Through elimination of the column purification and gel electrophoresis-based size-selection steps, the library construction workflow is amenable to automation.

Holly Baden-Tillson, JCVI scientist and project manager said: 'JCVI is very excited to be beta testing the SPRIworks Fragment Library System I. By implementing SPRIworks, we have significantly increased our throughput from six libraries to as many as 20 in a working day, with very consistent library size and recovery. This enhanced productivity will also help us to decrease our library construction costs.'

The automated benchtop system prepares up to 10 DNA libraries in five hours


'The SPRIworks system was designed to simplify and automate next-generation sequencing workflows,' said Patrick J. Finn Ph.D., director of research and development, Beckman Coulter Genomics. 'This will enable users to prepare more samples for sequencing, and the customer to fully utilise the growing capacity of next-generation sequencing systems, increase operational efficiency and maximise sequencing capability. 'We"re delighted to be working with JCVI during beta testing, and the performance data generated represents a major milestone in the development of the SPRIworks platform. We look forward to extending this collaboration with future SPRIworks applications.'

'Simplifying a complex genomics process such as fragment library construction for next-generation sequencing provides another example of SPRI technology application to sequencing sample preparation,' commented Julie Moore, director of marketing for Beckman Coulter Genomics. 'Working in close collaboration with our customers and genomic research leaders such as the JCVI allows us to better understand the challenges faced by scientists in the sequencing community and to develop robust solutions to their needs.'

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