New analyser for membrane protein stability tests

19-Feb-2015

The system reduces the time and cost of therapeutic protein pre-formulation studies

Avacta Analytical has extended the versatility of the Optim 2 protein stability and characterisation instruments, with a version featuring an additional 375nm laser that significantly enhances the measurement of stability information for membrane proteins.

'Approximately 30% of the human genome encodes membrane proteins, which include receptors, transport proteins and enzymes,' says Avacta’s Dr Geoff Platt, which means this product will be of particular interest to those in drug formulation and research.

The capability of the Optim 375 was recently evaluated in the Faculty of Biological Sciences at the University of Leeds. This work showed that while the use of intrinsic fluorescence to study the unfolding of membrane proteins is particularly effective for samples containing significant extra-membranous domains, for some proteins tryptophan is not a useful probe. However, the employment of a highly reactive thiol-specific fluorescent dye (CPM) with the Optim 375 allowed clear observation of unfolding for proteins containing buried cysteine residues, even at low concentrations (< 0.5 mg/ml) of the protein, making it a highly sensitive method.

These measurements were shown to work well for many membrane proteins that are otherwise difficult to characterise; particularly integral membrane proteins such as transporters, ion channels and G protein-coupled receptors.

The Optim 375 can be used to effectively guide purification and biophysical characterisation efforts, including crystallisation, by tracking the exposure of cysteine residues within the protein interior as an indicator of protein unfolding, with the ability to simultaneously follow protein aggregation using the system’s built-in static light scattering.

The work at the University of Leeds studied both the conformational thermal stability of the proteins and their propensity to aggregate and helped to demonstrate the advantages of the extra laser. It also highlighted the other benefits of Optim technology; ultra-low sample volumes, high-speed analysis and the ability to quickly and easily apply a range of different settings for each sample, by using Optim Client software.

Developed to reduce the time and cost of therapeutic protein pre-formulation studies, the Optim family of analysers are used in applications such as lead candidate selection and optimisation, candidate formulation and stability studies. The speed and cost advantages that these instruments deliver are due to the tiny sample volumes required (9 microlitres), and the ability to undertake parallel measurements of up to 48 samples in just a few hours.

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