Thermo Scientific adds Q Exactive BioPharma to portfolio


Enables three protein characterisation workflows on one instrument

Researchers performing biopharmaceutical characterisation can now benefit from a single system from Thermo Fisher Scientific that enables three workflows for the complete characterisation of biotherapeutics.

The new Thermo Scientific Q Exactive BioPharma MS/MS Hybrid Quadropole-Orbitrap mass spectrometer takes full advantage of the high-resolution, accurate-mass (HRAM) capabilities of the Orbitrap mass analyser to enable three key protein characterisation workflows; denatured and native MS intact analysis, subunit and top/middle-down analysis, and peptide mapping. Combined with recommended sample preparation and analysis methods based on Thermo Scientific reagents, columns, UHPLC and data processing technologies, the Q Exactive BioPharma constitutes a complete solution for the rigorous structural characterisation of biotherapeutic compounds, the firm says.

'We now offer a single instrument that encompasses all of our leading technologies into one system, which can be used to analyse antibody-based biologics at the intact, subunit, and peptide levels – ultimately providing an efficient route to high quality results,' said Jonathan Josephs, Director, pharma/biopharma marketing, life sciences mass spectrometry at Thermo Fisher.

We now offer a single instrument that encompasses all of our leading technologies into one system

When combined with Thermo Scientific BioPharma Finder Software, the technology is a powerful solution for biotherapeutic characterisation. The integrated workflow-driven interface enables automated processing of data from peptide, subunit, and intact protein analyses. BioPharma Finder uses two deconvolution algorithms to take full advantage of the ultra-high-quality, high-resolution accurate mass (HRAM) data produced by Orbitrap-based mass spectrometers to provide accuracy for intact mass measurements.

This software can also be used to accurately characterise disulfide bond linkages, detect low-level impurities and automate the relative quantitation and reporting of sequence variants and post-translational modifications.

To further complete the solution, the new Thermo Scientific Vanquish Flex Binary UHPLC system for pharmaceutical and biopharmaceutical laboratories performing qualitative and quantitative LC or LC-MS analysis adds a binary solvent delivery option in the 1000 bar (15,000psi) performance range.

The system is built for high-speed, fast gradient applications and the Vanquish platform’s outstanding retention time reproducibility, the firm says.

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Thermo Fisher is displaying these solutions at this week's (5–9 June) 64th American Society of Mass Spectrometry (ASMS) Conference on Mass Spectrometry and Allied Topics.

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