Enables three protein characterisation workflows on one instrument
Researchers performing biopharmaceutical characterisation can now benefit from a single system from Thermo Fisher Scientific that enables three workflows for the complete characterisation of biotherapeutics.
The new Thermo Scientific Q Exactive BioPharma MS/MS Hybrid Quadropole-Orbitrap mass spectrometer takes full advantage of the high-resolution, accurate-mass (HRAM) capabilities of the Orbitrap mass analyser to enable three key protein characterisation workflows; denatured and native MS intact analysis, subunit and top/middle-down analysis, and peptide mapping. Combined with recommended sample preparation and analysis methods based on Thermo Scientific reagents, columns, UHPLC and data processing technologies, the Q Exactive BioPharma constitutes a complete solution for the rigorous structural characterisation of biotherapeutic compounds, the firm says.
'We now offer a single instrument that encompasses all of our leading technologies into one system, which can be used to analyse antibody-based biologics at the intact, subunit, and peptide levels – ultimately providing an efficient route to high quality results,' said Jonathan Josephs, Director, pharma/biopharma marketing, life sciences mass spectrometry at Thermo Fisher.
We now offer a single instrument that encompasses all of our leading technologies into one system
When combined with Thermo Scientific BioPharma Finder Software, the technology is a powerful solution for biotherapeutic characterisation. The integrated workflow-driven interface enables automated processing of data from peptide, subunit, and intact protein analyses. BioPharma Finder uses two deconvolution algorithms to take full advantage of the ultra-high-quality, high-resolution accurate mass (HRAM) data produced by Orbitrap-based mass spectrometers to provide accuracy for intact mass measurements.
This software can also be used to accurately characterise disulfide bond linkages, detect low-level impurities and automate the relative quantitation and reporting of sequence variants and post-translational modifications.
To further complete the solution, the new Thermo Scientific Vanquish Flex Binary UHPLC system for pharmaceutical and biopharmaceutical laboratories performing qualitative and quantitative LC or LC-MS analysis adds a binary solvent delivery option in the 1000 bar (15,000psi) performance range.
The system is built for high-speed, fast gradient applications and the Vanquish platform’s outstanding retention time reproducibility, the firm says.
Thermo Fisher is displaying these solutions at this week's (5–9 June) 64th American Society of Mass Spectrometry (ASMS) Conference on Mass Spectrometry and Allied Topics.