Tough biogel structures produced by 3-D printing


Stretchable, biocompatible hydrogels with complex patterning could be used in tissue engineering

Researchers have developed a new way of making tough – but soft and wet – biocompatible materials, called 'hydrogels', into complex and intricately patterned shapes. The process might lead to injectable materials for delivering drugs or cells into the body; scaffolds for regenerating load-bearing tissues; or tough but flexible actuators for future robots, the scientists say.

The new process is described in the journal Advanced Materials, co-authored by MIT Associate Professor of Mechanical Engineering Xuanhe Zhao and colleagues at MIT, Duke University, and Columbia University in the US.

Zhao says the new process can produce complex hydrogel structures that are 'extremely tough and robust', and compatible with the encapsulation of cells in the structures. This could make it possible to 3D-print complex hydrogel structures, such as implants to be infused with cells and drugs and then placed in the body.

Hydrogels, defined by water molecules encased in rubbery polymer networks that provide shape and structure, are similar to natural tissues such as cartilage, which is used by the body as a natural shock absorber. The new 3-D printing process could eventually make it possible to produce tough hydrogel structures artificially for repair or replacement of load-bearing tissues, such as cartilage.

The innovation is really about the material – a new ink for 3-D printing of biocompatible tough hydrogel

While synthetic hydrogels are commonly weak or brittle, a number of tough and stretchable hydrogels have been developed over the last decade. However, previous ways of making them have usually involved 'harsh chemical environments' that would kill living cells encapsulated in them, says Zhao.

The new materials are benign enough to synthesise together with living cells — such as stem cells — which could then allow high viability of the cells, adds Zhao, who holds a joint appointment in MIT’s Department of Civil and Environmental Engineering.

The previous work was also not able to produce complex 3-D structures with tough hydrogels, Zhao says. The new biocompatible tough hydrogel can be printed into diverse 3-D structures such as a hollow cube, hemisphere, pyramid, twisted bundle, multilayer mesh, or physiologically relevant shapes, such as a human nose or ear.

The new method uses a commercially available 3D-printing mechanism, Zhao says. 'The innovation is really about the material – a new ink for 3-D printing of biocompatible tough hydrogel.' Specifically, a composite of two different biopolymers. 'Each [material] individually is very weak and brittle, but once you put them together, it becomes very tough and strong. It’s like steel-reinforced concrete,' he says.

One of the two polymers provides elasticity to the printed material, while the other allows it to dissipate energy under deformation without breaking. A third ingredient, a biocompatible 'nanoclay', makes it possible to fine-tune the viscosity of the material, improving the ability to control its flow through the 3D-printing nozzle.

The material can be made so flexible that a printed shape, such as a pyramid, can be compressed by 99%, and then spring back to its original shape.

Sungmin Hong, a lead author of the paper and a former postdoc in Zhao’s group, says it can also be stretched to five times its original size. Such resilience is a key feature of natural bodily tissues that need to withstand a variety of forces and impacts.

Such materials might eventually be used to custom-print shapes for the replacement of cartilaginous tissues in ears, noses, or load-bearing joints, Zhao says. Lab tests have already shown that the material is even tougher than natural cartilage.

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The next step will be to improve the resolution of the printer, which is currently limited to details about 500 micrometres in size, and to test the printed hydrogel structures in animal models.