Porvair Sciences has published an application report demonstrating how its Microlute CP Reverse Phase Solid Phase Extraction (SPE) microplates provide a robust and reproducible SPE LC-MS sample preparation methodology for various neutral, acidic, and basic analytes.
As a sample preparation method - solid phase extraction is acknowledged as a go-to tool for sample clean-up before analysis by hyphenated techniques such as LC/MS or GC/MS. Solid phase extraction offers less system downtime and troubleshooting, cleaner chromatograms with a reduction of contaminating compounds, and greater reproducible analyte recoveries. Unfortunately, traditional SPE methods rely upon loose-filled resins which often create problems such as voids in the sorbent beds leading to channelling and inconsistent flow-through of solutions. These undesirable effects lead to inconsistent results and poor analyte recovery.
Microlute CP SPE microplates from Porvair incorporate a polymer structure made up of an interconnected network of evenly distributed pores combined with retentive media. The hybrid technology ensures even liquid flow rates throughout the SPE process, which leads to sufficient time for Van der Waals forces of interaction to take place between the solid phase sorbent and the analytes resulting in a highly reproducible SPE method.
Experimental data is provided in the application report to show how Microlute CP RP 96 well microplates selectively retain and elute neutral, basic and acidic compounds. The data demonstrate how percentage relative standard deviation values from the Microlute CP are significantly lower than other commercial SPE plates on average. The note also demonstrates no analyte is lost in the load step of the SPE process leading to high recovery values. The Microlute CP RP 96 well-plate for solid phase extractions is also shown to offer significant benefits for the recovery of analytes.