AnaSpec adds HiLyte Fluor dyes to protein labelling kits
AnaSpec, a US provider of integrated proteomics solutions to biotech, pharmaceutical and academic research institutions, has added two new dyes to its AnaTag protein labelling kit collection.
AnaSpec, a US provider of integrated proteomics solutions to biotech, pharmaceutical and academic research institutions, has added two new dyes to its AnaTag protein labelling kit collection.
Called HiLyte Fluor 594 and HiLyte Fluor 680, these dyes span the visible spectrum and provide a way to label proteins using the succinimidyl ester (SE) reactive form of the HiLyte Fluor dyes.
The company said the succinimidyl ester shows good reactivity and selectivity with aliphatic amines of the protein and forms a carboxamide bond, which is identical to, and as stable as the natural peptide bond.
The HiLyte Fluor dyes can sustain treatments during immunofluorescent staining, fluorescence in situ hybridisation, flow cytometry and other biological applications without hydrolysis.
The kits can be used for microscale (3 x 200ug) and larger scale (3 x 5mg) labelling of proteins, such as antibodies. The labelling time can be as short as 30 minutes.