The first commercially available Automated Instrument for Cubic Phase in meso Protein Crystallography.
The Anachem Flexus is a highly flexible platform that provides complete automation for a wide variety of liquid handling applications. The latest model to be unveiled by Anachem is the only commercially available automated instrument for Cubic Phase in meso Protein Crystallography. Designed specifically for speed, precision and careful handling, the Flexus Crystal IMP embraces a technique that is otherwise difficult and laborious to perform manually.
One method for successful crystallisation of membrane proteins exploits the ability of lipids to form liquid crystals or mesophases. However, the method has not been widely adopted as it is difficult to perform. Problems include the need to manipulate an extremely viscous lipidic cubic phase and accurately positioning the precipitant on top of the cubic phase.
Now with the Flexus Crystal IMP the practical difficulties of delivering small nanolitre volumes of highly viscous cubic phase can be completely automated. The system uses a syringe mounted onto a fully integrated micro-syringe pump situated on the arm of the robotic platform. The mixed cubic phase is transferred directly to the micro-pump and precisely dispensed onto a glass slide, with precipitant added directly on top.
Plates can now be prepared in less than 10 minutes with excellent reproducibility, allowing high throughput trials that require minimal amounts of protein. The intense speed of this instrument now enables thorough screening of membrane proteins and allows users to concentrate on inspecting plates for crystals rather than in the preparation itself.
Anachem is the leading specialist distributor to the scientific communities within the UK and Ireland having over thirty five years of technical expertise and innovation in liquid handling and chromatography. Today, Anachem represents suppliers such as GE, Gilson, Munktell, RAININ, SSI and Treff, to provide world renowned manual liquid handling products, laboratory plastics, instrumentation and consumables backed by unprecedented quality of service and support.