NGSBIO Library Quant kit enables quantification across a range of sample types, concentrations, fragment sizes and GC content
PCR Biosystems has introduced the NGSBIO Library Quant kit for Illumina.
The kit contains components for the quantification of DNA libraries prior to next generation sequencing with Illumina platforms.
The Illumina system requires quantification of libraries prior to sequencing to ensure the correct amount of library is loaded into the machine. This is essential for generating the maximum amount of clustering data possible per run. An easier to see blue version of the kit has also been launched, containing a blue qPCR mix for greater pipetting precision and accuracy.
The kit contains 5 DNA standards, primers specific to the P5 and P7 Illumina adapter sequences, a library dilution buffer and qPCRBIO SyGreen Mix or qPCRBIO SyGreen Blue Mix. The blue qPCR mix contains a non-reactive dye to improve reaction mix visibility and aims to be useful when using small reaction volumes or white plates.
PCR also provide a free online calculation tool to help scientists analyse results generated with the kit. Users can download the results in a pdf or email the results to themselves and access the data online for up to 30 days.
Mark Stevens, Head of R&D said: "As specialists in PCR and other allied technologies, we listen closely to the needs of our customers, and we’ve developed our NGSBIO Library Quant Kit in response. qPCR is the most accurate technique available for library quantification, ensuring optimal cluster densities for improved sequencing efficiency and data quality. NGSBIO Library Quant Kit is designed to not only be highly accurate but also convenient and easy to use."
qPCR only measures adapter-bound molecules that can be used as templates for library amplification and cluster generation, as opposed to the quantity of all double stranded DNA that is present.