Scientists from Oxford University’s Department of Physics say they have developed a diagnostic test that detects and identifies viruses in less than five minutes.
The method, published on the preprint server MedRxiv, is able to differentiate SARS-CoV-2 from negative clinical samples, as well as from other common respiratory pathogens such as influenza and seasonal human coronaviruses.
Working on throat swabs from COVID-19 patients, without the need for genome extraction, purification or amplification of the viruses, the method starts with labelling of virus particles in the sample with short fluorescent DNA strands. A microscope is then used to collect images of the sample, with each image containing hundreds of fluorescently labelled viruses.
Machine learning software quickly and automatically identifies the virus present in the sample. This approach exploits the fact that distinct virus types have differences in their fluorescence labelling due to differences in their surface chemistry, size and shape.
The scientists have worked with clinical collaborators at the John Radcliffe Hospital in Oxford to validate the assay on COVID-19 patient samples which were confirmed by conventional RT-PCR methods.
They are currently working with Oxford University Innovation (OUI) and two external business/finance advisors to set up a spinout, and are seeking investment to accelerate the translation of the test into a integrated device to be deployed as a real-time diagnostic platform capable of detecting multiple virus threats.
Professor Achilles Kapanidis, at Oxford’s Department of Physics, says: ‘Unlike other technologies that detect a delayed antibody response or that require expensive, tedious and time-consuming sample preparation, our method quickly detects intact virus particles; meaning the assay is simple, extremely rapid, and cost-effective.’
DPhil student Nicolas Shiaelis, at the University of Oxford, says: ‘Our test is much faster than other existing diagnostic technologies; viral diagnosis in less than 5 minutes can make mass testing a reality, providing a proactive means to control viral outbreaks.’
Dr Nicole Robb, formally a Royal Society Fellow at the University of Oxford and now at Warwick Medical School, says: ‘A significant concern for the upcoming winter months is the unpredictable effects of co-circulation of SARS-CoV-2 with other seasonal respiratory viruses; we have shown that our assay can reliably distinguish between different viruses in clinical samples, a development that offers a crucial advantage in the next phase of the pandemic.’