QTOF mass spectrometry for faster biotherapeutics characterisation

Published: 22-Mar-2017

Sciex focuses on reproducible data and education for biologics labs with the X500B QTOF at World ADC Summit

Global life science analytical technologies company Sciex featured the X500B Quadrupole Time-of-Flight (QTOF) System at the 7th World ADC Summit, in Berlin, Germany.

The new technology provides biotherapeutics characterisation that makes quantitative analysis accessible to pharmaceutical laboratories of all sizes.

In the midst of increasing regulatory requirements for biotherapeutics development and manufacturing, scientists are continually looking for ways to improve the quality, reliability and reproducibility of characterisation data.


The X500B QTOF System was specifically developed to deliver ease-of-use for high resolution standardised workflows for biotherapeutic developers.

The system includes BioPharmaView 2.0 processing software that simplifies data processing. The software can also be used to interpret SWATH 2.0 data independent acquisition on the X500B QTOF System.

SWATH Acquisition allows peptide mapping data for every peptide in the sample from a single injection, including low-abundance peptides and post-translational modifications.

Other systems

Another Sciex solution featured at the World ADC Summit included the PA 800 Plus Pharmaceutical Analysis System, which provides automated quantitative analysis of biotherapeutics for development and quality control processes.

The capillary electrophoresis system provides QA/QC for capillary electrophoresis applications, including characterisation of product purity, charge heterogeneity and glycan analysis.

Glycan analysis has recently been enhanced with the launch of the Fast Glycan Labeling and Analysis Kit, which can run glycans up to five times faster than traditional HILIC approaches.

Also at the Summit

Sciex presented a seminar entitled “The simpler and faster LC-MS solution for routine biopharmaceutical characterisation”.

The seminar gave insights into improving ADC characterisation. Topics included ways to improve data quality while simplifying data collection and recent technology developments that have made mass spectrometry more accessible to routine biotherapeutics analysis.

Sciex also held a workshop, which included a presentation from Professor Yannis Francois on CESI-MS characterisation of ADCs.

In this presentation, Francois highlighted the use of the CESI 8000 Plus System for high-order structural interrogation of antibody-drug conjugates by a combination of intact, middle-up and bottom-up techniques using CESI-MS.

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